AntagomiR-27a Targets FOXO3a in Glioblastoma and Suppresses U87 Growth in Vitro and in Vivo
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چکیده
Glioblastoma (GBM) is one of the most common intracranial tumors, about 60% of caes being malignant. As there is no effective treatment for glioblastoma, even combined surgery, radiotherapy and chemotherapy, the median survival period of glioblastoma patients is about 9-12 months. With the development of molecular biology a few genes, including oncogene and tumor suppressor gene, were found to be associated with the development and progression of glioblastoma, however, the underlying mechanism remains unclear (Burgess et al., 2008). With the improvement of studies on miRNA, more and more evidences indicate that miRNA might play a great role in the development and progression of glioblastoma , thus providing new insights by developing miRNA-based methods to diagnose and treat glioblastoma (Sevignani et al., 2006; Papagiannakopoulos et al., 2008). MicroRNAs (miRNAs) are endogenous non-coding short chain RNAs with a length of about 22nt and they are highly conserved and widely expressed in the plant and animal cell. By binding to target sites in the 3’-UTR of target mRNAs, miRNAs inhibit the expression of target genes (Cummins et al., 2006). MiR-27, including miR27a and miR-27b, was firstly cloned form Hela cells by Mourelatos in 2002 (Mourelatos et al., 2002). It has been reported that miR-27a played as oncogene in pancrease
منابع مشابه
AntagomiR-27a targets FOXO3a in glioblastoma and suppresses U87 cell growth in vitro and in vivo.
OBJECTIVE To study the effect of the antagomiR-27a inhibitor on glioblastoma cells. METHODS The miR- 27a expression level in specimens of human glioblastoma and normal human brain tissues excised during decompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27a was screened out through bioinformatics databases, and the predicted gene was verified...
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تاریخ انتشار 2013